5X SDS-PAGE Sample Loading Buffer
250mM Tris-HCl, pH 6.8, 0.5M DTT, 10% SDS, 50% Glycerol, 0.2% Bromophenol blue.
PRODUCT DETAILS
Product Description
CELLPICK 5X SDS-PAGE Sample Loading Buffer is an optimized, ready-to-use formulation designed for the preparation of protein samples prior to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Formulated with Tris-HCl, SDS, glycerol, DTT, and bromophenol blue, it ensures complete protein denaturation, reduction of disulfide bonds, and proper tracking during electrophoresis. Supplied in a convenient 1 mL × 10 format to prevent freeze-thaw degradation and maintain stability.
Specifications
Recommended Applications
Used for denaturing protein samples for standard Laemmli SDS-PAGE and Western blotting applications.
Usage Instructions
Mix 1 volume of 5X Loading Buffer with 4 volumes of protein sample. Heat at 95–100°C for 5 minutes prior to loading onto the gel.
Ordering Information
Catalog Number
Product Name
Packaging Unit
5X SDS-PAGE Sample Loading Buffer
S003-1MLX10
1ml x 10
DATASHEET
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