16% Paraformaldehyde Solution
16% Paraformaldehyde in PBS.
PRODUCT DETAILS
Product Description
CELLPICK 16% Paraformaldehyde (PFA) Solution is an ultra-concentrated, EM-grade fixative prepared in standard 1X Phosphate-Buffered Saline (PBS). This extremely robust master stock solution provides laboratories with maximum flexibility to formulate highly customized fixative concentrations (e.g., 8%, 4%, or 2%) required for specialized morphological studies, tissue perfusion, and electron microscopy.
By utilizing this pre-mixed, stabilized 16% formulation, researchers entirely avoid the severe inhalation hazards and extensive time associated with heating and depolymerizing raw PFA powder. The 500 mL format offers an exceptionally concentrated and economical resource for core facilities and high-demand research settings.
Specifications
Recommended Applications
16% PFA is designed as a potent master stock for preparing working fixatives. It is essential for Electron Microscopy (EM), specialized Immunohistochemistry (IHC), and complex in vivo tissue perfusion protocols requiring high initial cross-linking density.
Usage Instructions
To prepare a standard 4% PFA working solution, dilute the 16% PFA stock 1:4 with sterile 1X PBS (e.g., 1 mL of 16% PFA + 3 mL of PBS) immediately before application. For standard cell fixation, apply the diluted 4% fixative to washed cells and incubate for 10–20 minutes at room temperature, followed by thorough PBS washing to remove residual fixative.
Ordering Information
Catalog Number
Product Name
Packaging Unit
16% Paraformaldehyde Solution
P004-0500
500ml
DATASHEET
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