0.5M EGTA, pH 8.0
500mM Na2EGTA, pH adijusted to 8.0 with sodium hydroxide, sterile solution.
PRODUCT DETAILS
Product Description
CELLPICK 0.5M EGTA, pH 8.0 is a premium, ready-to-use chelating agent specifically designed for the highly selective sequestration of calcium ions (Ca2+). Unlike standard EDTA, EGTA exhibits a significantly lower affinity for magnesium ions (Mg2+), making it the reagent of choice for protocols where magnesium-dependent enzymatic activity (such as ATP-dependent reactions) must be preserved while inhibiting calcium-dependent processes.
Because EGTA powder is notoriously difficult to dissolve, requiring time-consuming pH adjustments, this sterile, pre-mixed liquid formulation eliminates tedious preparation steps and ensures maximum consistency and reproducibility across sensitive biological assays.
Specifications
Recommended Applications
This highly specialized buffer is frequently used in cell biology and biochemistry workflows. Key applications include the preparation of specific enzyme assay buffers (e.g., adenylate cyclase and calcium-dependent protein kinases), in vivo intracellular calcium signaling studies, and specialized protein extraction protocols.
Usage Instructions
Add the appropriate volume directly to your working solutions, cell culture media, or extraction buffers to achieve the desired final EGTA concentration. No further heating, stirring, or pH adjustment is required before use.
Ordering Information
Catalog Number
Product Name
Packaging Unit
0.5M EGTA, pH 8.0
E004-0100
100ml
DATASHEET
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