Destain Solution
45% Methanol, 10% Glacial Acetic Acid, Solution.
PRODUCT DETAILS
Product Description
CELLPICK Destain Solution is a ready-to-use, optimized solvent mixture formulated specifically for the rapid removal of unbound background stain from polyacrylamide gels following protein visualization. Composed of a precise blend of 45% methanol and 10% glacial acetic acid, this solution effectively washes away excess dye, significantly enhancing the contrast and visibility of protein bands.
By utilizing a pre-mixed liquid formulation, it eliminates the need for handling concentrated and hazardous solvents in the laboratory, thereby saving valuable preparation time and ensuring highly reproducible destaining performance across all experiments.
Specifications
Recommended Applications
This solution is primarily designed for the rapid destaining of standard SDS-PAGE and native PAGE gels previously stained with traditional Coomassie Blue formulations, such as R-250.
Usage Instructions
Following the staining procedure, completely submerge the gel in a generous volume of Destain Solution. Agitate gently on an orbital shaker at room temperature. For optimal results, discard the used solution and replace it with fresh Destain Solution periodically (e.g., every 30–60 minutes) until the gel background is completely clear and the protein bands exhibit high contrast. To prevent gel shrinkage during long-term storage, transfer the gel to a weak acetic acid solution (e.g., 7%) or distilled water after complete destaining.
Ordering Information
Catalog Number
Product Name
Packaging Unit
Destain Solution
D001-1000
1000ml
DATASHEET
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