Coomassie Blue G-250 Stain
10% Acetic acid, 0.1% Coomassie blue G-250, Stain solution.
PRODUCT DETAILS
Product Description
CELLPICK Coomassie Blue G-250 Stain is a ready-to-use, colloidal staining solution designed for fast, highly sensitive, and convenient protein detection in polyacrylamide gels. Its optimized formulation ensures exceptional signal-to-noise ratios and clean backgrounds.
Unlike traditional R-250 stains, this colloidal G-250 formulation selectively binds to proteins rather than the gel matrix, virtually eliminating the need for prolonged destaining steps. It delivers high sensitivity, capable of detecting as little as 8–10 ng of protein per band, while saving preparation time and ensuring reproducibility across experiments.
Specifications
Recommended Applications
This product is extensively used for protein visualization and quantification following electrophoresis. Key applications include standard SDS-PAGE protein visualization, two-dimensional (2D) gel electrophoresis, and post-electrophoresis protein quantification. It is also compatible with downstream mass spectrometry (MS) analysis.
Usage Instructions
Following electrophoresis, immerse the gel in sufficient staining solution and agitate gently at room temperature for 1–2 hours. Destaining is typically not required; however, washing the gel with deionized water or a weak acetic acid solution can further enhance background clarity.
Ordering Information
Catalog Number
Product Name
Packaging Unit
Coomassie Blue G-250 Stain
C001-1000
1000ml
DATASHEET
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