Sodium Bicarbonate, 7.5%

Sodium Bicarbonate (NaHCO₃) is a representative substance used to maintain the pH of culture media during cell culture in an environment where the CO₂ concentration in the air is 4~10%. Sodium Bicarbonate has almost no cytotoxicity and is included in most cell culture media. The usage concentration of Sodium Bicarbonate varies depending on the type of medium: DMEM is 3.7 g/L, MEM, Alpha-MEM, M199, and McCoy's 5A are 2.2 g/L, RPMI1640 is 2.0 g/L, and DMEM/F12 is 1.2 g/L. This product (Cat. No. CSDBC0012) is a high-concentration solution of 7.5% (75.0 g/L) and should be diluted to an appropriate concentration depending on the cell type and culture conditions.