PRODUCT DETAILS
Product Description
DMEM/F12 Medium is a 1:1 mixture of DMEM and Ham's F12, containing not only the high concentrations of glucose, amino acids, and vitamins found in DMEM, but also various components of Ham's F12. It is used for culturing various cells such as MDCK, glial cells, fibroblasts, and human endothelial cells. Because Ham's F12 Nutrient Mixture itself was developed as a serum-free medium, DMEM/F12 is widely used as a basal medium for culturing cells under reduced serum or serum-free conditions. This product (Cat. No. DF12-007) contains 15 mM HEPES for a more effective pH buffering effect. In addition, L-Glutamine is not included and must be added as needed.
Composition
Product Quality Specifications
Storage Conditions and Precautions
Store at refrigerated temperatures (2~8°C) protected from direct light. The expiration date is indicated on the product label; do not use expired products. Product degradation may appear as precipitates, floating particles, turbidity, or color change; discard if any are observed. Storing against the inner walls of a refrigerator where temperatures may approach 0°C can cause degradation due to ice crystal formation. The addition of extra substances may also cause degradation.
Usage Instructions and Precautions
This product is a sterile solution. Open the cap only in a sterile environment (e.g., a clean bench) using aseptic technique. It is recommended to use the entire contents after opening; otherwise, aliquot into a suitable sterile container and store refrigerated (2~8°C).
DATASHEET
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