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DMEM (High) (HEPES)

With 4.5 g/L D-Glucose
With L-Glutamine
With 25 mM HEPES
With Sodium Bicarbonate

PRODUCT INFORMATION

CAT NO.

SIZE

CPD003

500ml

PRODUCT DETAILS

Product Description

DMEM is a modification of Basal Medium Eagle (BME), containing a fourfold higher concentration of amino acids and vitamins. Originally developed for embryonic mouse cells with 1.0 g/L D-Glucose, formulations with 4.5 g/L D-Glucose are widely used for culturing a broad range of cell lines including HeLa, 293, Cos-7, and PC-1, as well as primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. This product contains 25 mM HEPES to provide more effective pH buffering capacity.

Composition

Product Quality Specifications

Storage Conditions and Precautions

Protect from direct light and store refrigerated (2–8°C). Product degradation may appear as precipitates, floating particles, turbidity, or color change; discard if any are observed. Storing against the inner walls of a refrigerator where temperatures may approach 0°C can cause degradation.

Usage Instructions and Precautions

This product is a sterile solution. Open the cap only in a sterile environment (e.g., a clean bench) using aseptic technique. It is recommended to use the entire contents after opening; otherwise, aliquot into a suitable sterile container and store refrigerated (2–8°C).

DATASHEET

Product Datasheet

PDF Document

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Contact

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