ITSP, 100X

ITSP Solution is a mixture of insulin, transferrin, selenium, and pyruvate dissolved in EBSS (Earle's Balanced Salt Solution, without phenol red). It promotes the growth of various adherent cells when serum such as FBS is used at low concentrations (0~4%) in the basal culture medium during cell culture. Insulin is involved in the intracellular uptake of glucose and amino acids, lipid synthesis, ion transport, and the synthesis of proteins and nucleic acids. Transferrin acts as an iron carrier and reduces the toxicity of oxygen radicals and peroxides. Selenite acts as a co-factor for various proteins and provides antioxidant functions in the culture medium. Pyruvate plays a role in generating energy through the Krebs cycle and is an important precursor for the synthesis of amino acids, fatty acids, and cholesterol. This product is a 100-fold concentrated solution and is used by diluting it 1/100 in the basal culture medium (e.g., adding 10 mL of ITSP, 100X to 1 L of basal culture medium). In particular, the insulin and transferrin contained in this product are raw materials manufactured using recombinant protein technology, making them suitable for cultures requiring animal component-free conditions.