PRODUCT DETAILS
Product Description
HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) has a pKa (25°C) of 7.48 and an effective pH range of 7.0 to 8.0, making it a widely used buffer in cell culture. It is especially excellent for stably and consistently maintaining the pH of culture media regardless of changes in CO2 concentration as cells grow. Therefore, culture media containing a certain amount of HEPES can maintain pH more stably during the cell culture period compared to media containing only sodium bicarbonate (NaHCO3), which is frequently used in cell culture.
Composition
Product Quality Specifications
Storage Conditions and Precautions
Protect from direct light and store refrigerated (2~8°C). The expiration date is indicated on the product label; do not use expired products. Product degradation may appear as precipitates, floating particles, turbidity, or color change; discard if any are observed. Storing against the inner walls of a refrigerator where temperatures may approach 0°C can cause degradation due to ice crystal formation. The addition of extra substances may also cause degradation.
Usage Instructions and Precautions
This product is a sterile solution. Open the cap only in a sterile environment (e.g., a clean bench) using aseptic technique. It is recommended to use the entire contents after opening; otherwise, aliquot into a suitable sterile container and store properly.
DATASHEET
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