L-Glutamine, 200 mM

L-Glutamine is an essential amino acid required for cell culture. It is necessary for the synthesis of nucleotides, amino sugars, glutathione, and L-glutamate, and plays a vital role in protein synthesis, gluconeogenesis, and the production of intracellular nitrogen compounds. While the optimal concentration varies depending on cell and medium types, it is generally used at around 2 to 8 mM. Although relatively stable in powder or frozen forms, L-Glutamine is unstable in solution and degrades faster than other amino acids, producing ammonia that can be toxic to cells, and therefore must be handled with care.